Biochemical, Structural and Physiological Characteristics of Vacuolar H+-Pyrophosphatase
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چکیده
منابع مشابه
Acidocalcisomes and a vacuolar H+-pyrophosphatase in malaria parasites.
Plasmodium berghei trophozoites were loaded with the fluorescent calcium indicator, fura-2 acetoxymethyl ester, to measure their intracellular Ca(2+) concentration ([Ca(2+)](i)). [Ca(2+)](i) was increased in the presence of the sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase inhibitor, thapsigargin. Trophozoites also possess a significant amount of Ca(2+) stored in an acidic compartment. This ...
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The H(+)-pyrophosphatase (H(+)-PPase) consists of a single polypeptide, containing 16 or 17 transmembrane domains. To determine the higher order oligomeric state of Streptomyces coelicolor H(+)-PPase, we constructed a series of cysteine substitution mutants and expressed them in Escherichia coli. Firstly, we analyzed the formation of disulfide bonds, promoted by copper, in mutants with single c...
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Vacuolar proton-translocating inorganic pyrophosphatase and H(+)-ATPase acidify the vacuoles and power the vacuolar secondary active transport systems in plants. Developmental changes in the transcription of the pyrophosphatase in growing hypocotyls of mung bean (Vigna radiata) were investigated. The cDNA clone for the mung bean enzyme contains an uninterrupted open reading frame of 2298 bp, co...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2018
ISSN: 0032-0781,1471-9053
DOI: 10.1093/pcp/pcy054